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Jun 9, 2022 · RNA interference of other H3-H4 chaperones or H3 or H4 histones also rescued H2B 3KO sterility. We showed that blocking H3-H4 chaperones recovered cell division in C. elegans carrying the oncohistone H2BE74K mutation that distorts the H2B-H4 interface and induces nucleosome instability. Our results indicate that reducing chromatin H3-H4 rescues ...
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We derived haESC3KO with quadruple frameshift mutations at Sfmbt2,Jade1,Smoc1,andGab1usingCRISPR-Cas9-mediated gene editing (D4-haESC3KO)(Figures S1D–S1H). After MII oocyte injectionofD4-haESC3KO,thereconstructedembryos(D4-embry-o3KO) were transferred into the oviduct of pseudopregnant mice (Figure 1E). However, development of D4-embryo3KO was
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